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KMID : 0364819980340030169
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Korean Journal of Microbiology
1998 Volume.34 No. 3 p.169 ~ p.174
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Seasonal Changes of bacterial community analysed by fluorescent in situ hybridization method in Lake Soyang
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Hong Sun-Hee
Ahn Tae-Seok
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Abstract
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To define the structure and diversity of bacterial communities in the aqutic ecosystem, Lake Soyang, the largest artificial reservoir in Korea, a new method, fluorescent in situ hybridization was applied. This technique relies on the specific hybridization of the nucleic acid probes to the naturally amplified intracellular rRNA By this method, the bacterial community composition of Lake Soyang and bacterial numbers belong to eubacteria, proteobacteria and Cyrophaga-Flavobacterium group were estimated. Total bacterial numbers ranged from 0.3X 1062.0 X 106 cells ? ml-´, and vertical profile of total bacteria showed the peak at 2 and 5 m depths. The ratio of eubacteria to total bacteria were 22-100% and varied with depth and season. The percentage of Proteobacteria agroup ranged 2.6-66.7%, a-group 4.5-53.5%, y-group 4.6-76.7% and Cytophaga-Flavobacterium group 2.135.9%. Also, bacterial community had spatial and temporal characteristics. The dominant groups were ri-group in winter, y-group in spring and early summer and a-group in summer.
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KEYWORD
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in situ hybridization, bacrerial community, probe, Lake Soyang
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